Budesonide Down-regulates IL-1beta-Mediated MUC2/MUC5AC Genes Expression and Mucin Secretion in Human Airway Epithelial Cells / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 873-877, 2002.
Artículo
en Coreano
| WPRIM
| ID: wpr-651652
ABSTRACT
BACKGROUND AND OBJECTIVES:
Mucus hypersecretion is a hallmark of many respiratory inflammatory diseases such as asthma, bronchitis and sinusitis. While the current therapeutic pharmacological approaches to reducing mucus hypersecretion are limited, clinical studies have suggested that glucocorticoids reduce mucus secretion in patients with airway disease. However, the effect of glucocorticoids on mucus hypersecretion is not clear. Recently, we observed that IL-1beta induces MUC2 gene expression and mucin secretion in a previous experiment. This study was designed to investigate the effects of budesonide on the IL-1beta-mediated MUC2/5AC genes expression and mucin secretion. MATERIALS ANDMETHOD:
We observed the steady state mRNA level of MUC2/5AC genes using RT-PCR and mucin protein using immunoassay method in cultured human airway NCI-H292 epithelial cells.RESULTS:
Budesonide attenuated IL-1beta-mediated MUC2/5AC gene expression as well as mucin secretion. The attenuated effect of budesonide was in a dose-dependent pattern. This attenuated effect of budesonide was blocked by glucocorticoid receptor antagonist, RU-486.CONCLUSION:
This result suggests that budesonide suppresses the IL-1beta-mediated MUC2/5AC genes expression and mucin secretion via blockage of glucocorticoid receptor.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Asma
/
Sinusitis
/
Bronquitis
/
ARN Mensajero
/
Inmunoensayo
/
Receptores de Glucocorticoides
/
Expresión Génica
/
Mifepristona
/
Budesonida
/
Células Epiteliales
Límite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Otolaryngology - Head and Neck Surgery
Año:
2002
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS