The Effect of Hyaluronic Acid on the Expression of MMP-13 in Healing Process of Bone Defect in Rats / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
;
: 1021-1027, 2006.
Artículo
en Coreano
| WPRIM
| ID: wpr-653218
ABSTRACT
PURPOSE:
Matrix metalloproteinase (MMP)-13 has a degradation effect on type II collagen and is expressed in osteoblastic cells and hypertrophic chondrocytes. However, the role of MMP-13 is not completely understood. This study investigated the effect of exogenous Hyaluronic acid (HA) on the expression of MMP-13 in the healing process of bone defects. MATERIALS ANDMETHODS:
After making a bone defect in the metaphysis of the distal femur in rats, HA was instilled into the defect in the experimental group. In the control group, a bone defect was made but HA was not instilled. Two weeks after the experiment, the rats were sacrificed and the callus that filled the bone defects was obtained. A histological examination and real time reverse transcription polymeric chain reaction (RT-PCR) were performed to investigate the healing response and expression of MMP-13.RESULTS:
Histologically, the proliferation of osteoblasts and the formation of osteoid were more active in the experimental group. Nevertheless, the level of MMP-13 expression was significantly lower in the experimental group than in the control group.CONCLUSION:
From the results of this study, it appears that exogenous HA inhibits the expression of MMP-13 at the early stage of bone healing.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Polímeros
/
Callo Óseo
/
Condrocitos
/
Colágeno Tipo II
/
Transcripción Reversa
/
Fémur
/
Ácido Hialurónico
Límite:
Animales
Idioma:
Coreano
Revista:
The Journal of the Korean Orthopaedic Association
Año:
2006
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS