Clinical Significance of the Detection of Human Papillomavirus using DNA Chip Microarray Method In Respiratory Papilloma / 대한이비인후과학회지

ABSTRACT

BACKGROUND AND OBJECTIVES: Human Papillomavirus (HPV) is detected in the great majority of respiratory papillomas (RP). The current study was designed to investigate the significance of the presence and the type of HPV DNA and their relationship with the clinical course in RP. SUBJECTS AND METHOD: A total of 65 patients diagnosed with RP by pathologic examinations at the Asan medical center between July, 1990 and April, 2003 were included. There were 43 males and 22 females, with the mean age of 39.8+/-8.7 years (range 1 to 64 yrs). Of these, 33 patients were treated for the recurrent RPs the mean follow up period was 22.5 months. HPV DNA and the type were examined using paraffin embedded tissue by the DNA chip method. RESULTS: HPV DNA was detected in the tissue samples in 52 cases (80%); HPV types 16, 6, and type 11, in the order of frequency. Of the 33 recurrent RPs, HPV was detected in 31 cases (93.9%). Multiple infections observed in 23 patients, was more frequent in patients with 4 or more operations (54%) than in those with 3 or less operations (41%) (p value=0.193). While viral types remained the same in most cases of RPs with single HPV type infections, the HPV types changed in the majority of the cases of RPs with multiple HPV infections. The type of HPV was not correlated with the recurrence rate. CONCLUSION: This study confirms the presence of HPV in the great majority of RP in accordance with the literature, and demonstrates the utility of the DNA chip method. The most frequent HPV type was 16, in contrast to the types 6 and 11 of the previous studies. There was a trend for higher rates of multiple infections among recurred cases (p=0.193). During recurrences, HPV viral types changed in the majority of RPs with multiple HPV infections. HPV type did not show any correlation with recurrences.