The Influence of Fibronectin and/or RGDS Tetrapeptide on Osteopontin Expression in Cultures of Rat Calvarial Osteoblasts / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
; : 145-151, 2002.
Article
en Ko
| WPRIM
| ID: wpr-654553
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: To investigate the effect of fibronectin (FN) and/or RGDS tetrapeptide on osteoblastic proliferation and osteopontin expression. MATERIALS AND METHODS: Osteoblasts were cultured on tissue culture polystyrene (TCPS) and apatite-wallastonite glass ceramics (AW-GC) with or without FN and RGDS synthetic tetrapeptide. Osteoblastic proliferation and differentiation were measured after cultivation, by determining the number of attached cells, by [3H]-thymidine assay, and by measuring the activity of alkaline phosphatase. The expression of osteopontin (OPN) was determined by RT-PCR and western blotting. RESULTS: Cellular proliferation was more increased by FN than the other variables examined (P<0.03). OPN mRNA expression by RT-PCR was induced two-fold, 24-hour after FN treatment (P<0.05). The expression of OPN by western blotting showed a five-fold increase in cells treated with FN compared with the controls. The synthetic RGDS peptides partially blocked the growth of cells induced by FN (P<0.05). RGDS tetrapeptide alone increased the OPN expression induced by cultured osteoblasts (P<0.05). CONCLUSION: FN enhanced osteoblastic proliferation on glass ceramics and induced OPN expression. Integrin occupancy by RGD-containing molecules may play a role in the process of osteoblastic proliferation rather than in osteoblastic differentiation.
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Índice:
WPRIM
Asunto principal:
Osteoblastos
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Péptidos
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Poliestirenos
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ARN Mensajero
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Cerámica
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Western Blotting
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Fibronectinas
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Proliferación Celular
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Fosfatasa Alcalina
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Osteopontina
Límite:
Animals
Idioma:
Ko
Revista:
The Journal of the Korean Orthopaedic Association
Año:
2002
Tipo del documento:
Article