Development and Verification of Nested PCR Assay for Detection of Tobacco rattle virus in Plant Quarantine
Journal of Bacteriology and Virology
;
: 54-61, 2015.
Artículo
en Coreano
| WPRIM
| ID: wpr-65496
ABSTRACT
Tobacco rattle virus (TRV) is a plant pathogen belonging to the Group IV positive-sense single-stranded RNA viruses. TRV causes disease in various plants (e.g., potato, tomato and tobacco), for which it was classified as a controlled quarantine virus in Korea. This study aimed to develop specific primer sets for the rapid detection of TRV. Two RT-PCR primer sets were developed for specific detection of TRV. Furthermore, nested primer sets were also developed, which is required for high sensitivity detection in plant quarantine. The RT-PCR and nested PCR products had the following sizes set 5 (1,096-->540 bp), and set 7 (878-->756 bp), respectively. In addition, a modified positive-control plasmid was also developed for use as a positive control in TRV quarantine. The diagnostic system for TRV detection was verified using samples from Korean quarantine sites for the last five years (2009-2014). A total of 83 cases were detected among various import crops. This system for detection of TRV will continuously contribute to plant quarantine in the future.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas
/
Plásmidos
/
Virus ARN
/
Nicotiana
/
Solanum tuberosum
/
Cuarentena
/
Reacción en Cadena de la Polimerasa
/
Solanum lycopersicum
/
Corea (Geográfico)
Tipo de estudio:
Estudio diagnóstico
País/Región como asunto:
Asia
Idioma:
Coreano
Revista:
Journal of Bacteriology and Virology
Año:
2015
Tipo del documento:
Artículo
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