Establishment of canine kidney cell line for canine distemper virus replication / 동물의과학연구지
Journal of Biomedical Research
;
: 6-12, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-65508
ABSTRACT
Kidney cells of canine embryos were separated into single cells using collagenase and dispase. Primary culture was conducted using these cells. To remove fibroblasts, these cells were treated with edetate disodium dihydrate (Na2EDDA), and pure epithelial cells were separated. Recombinant retrovirus particles that manifest teromerase were produced and inoculated into primary culture cells to produce immortalized canine cell strains (JNUCK-1 and JNUCK-2). To examine the characteristics of the produced cell strains, the growth curve, maximum cultured households, and expressed proteins (keratin) were identified. The JNUCK-1 and JNUCK-2 cell lines showed division ability until the 30th generation without growth retardation. JNUCK-1 and JNUCK-2 cell lines clearly expressed telomerase until the 25th generation. The canine distemper virus (CDV) was inoculated into the JNUCK-1 and JNUCK-2 cell lines, as well as in the Madin-Darby canine kidney (MDCK) cell line. The maximum titer of CDV from the JNUCK-1 cell strain was about 200 times higher than that from the MDCK cell strain. However, the JNUCK-2 cell strain produced a lower titer than the MDCK cell strain. We established a new canine kidney epithelial cell line (JNUCK-1) that could produce CDV with high titer.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Retroviridae
/
Línea Celular
/
Composición Familiar
/
Colagenasas
/
Telomerasa
/
Virus del Moquillo Canino
/
Estructuras Embrionarias
/
Células Epiteliales
/
Fibroblastos
/
Células de Riñón Canino Madin Darby
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Journal of Biomedical Research
Año:
2015
Tipo del documento:
Artículo
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