The Effects of Bear Bile on the Expression of the p53 Protein in Cancer Cell Lines / 대한해부학회지

ABSTRACT

The effects of bear bile on the expression of the p53 protein in cancer cell lines were investigated by Western blot and RT-PCR methods. The p53 protein expression was increased after addition of bear bile in HaCaT, KUMA3, KUMA4 and KUMA6 cell lines that carried mutations n the p53 gene, but it is not ncreased n HCT116 and KUMA5 cell lines with the normal p53 gene as determined by Western blot method. The level of p53 mRNA was not altered in all cell lines after the treatment of bear bile as determined RT-PCR. These results indicate that the increment of the p53 protein after addition of bear bile was caused by increase in the protein stability but not by increase in protein synthesis. It can be concluded that bear bile affects positively only in the cell lines which have genetic variation of the p53 gene, and does not affect in the cell lines which have wild-type p53 gene, on the other hand.