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Detection of Fungus and Bacteria in Otitis Media with Effusion of Children Using Polymerase Chain Reaction and Its Correlation of Clinical Factors / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery ; : 960-966, 2008.
Artículo en Coreano | WPRIM | ID: wpr-656250
ABSTRACT
BACKGROUND AND

OBJECTIVES:

Several hypotheses have been proposed in the etiopathogenesis of otitis media with effusion (OME). The bacterial or fungal infection may also play a major role in the pathogenesis of OME. To investigate the relationship between bacteria and fungi as pathogens in OME and to analyze the incidence, the authors evaluated the pathogens of OME using polymerase chain reaction (PCR) technique, which is known to be more sensitive and specific than conventional bacterial and fungal culture. SUBJECTS AND

METHOD:

Twenty-three children (thirty ears), who were diagnosed with OME and had underwent ventilation tube insertion, were evaluated in the department of ORL-HNS, University Hospital, from May 2006 to March 2007. The middle ear effusion, obtained during the procedure, was evaluated for the identification of bacteria and fungi by PCR.

RESULTS:

Among 30 ears, viable pathogenic bacteria were detected in 20 ears (66.6%) and fungi in 13 ears (43.3%). The bacterial pathogens included Haemophilus influenzae (13 ears), followed by Streptococcus pneumonize (6 ears). The fungi detected from PCR were Candida albicans (4 ears-30.8%), Aspergillus niger (2 ears-15.4%) and Paecilomyces lilacinus (2 ears-15.4%). Additional pathogens include Basidiomycete yeast, Saccharamycete sp., Eurotium rubrum, Dothioraceae sp. and Stemphylium solani. Detection of fungal DNA was more common in patients with cleft palate and in recurrent cases with statistical significance.

CONCLUSION:

The use of PCR of middle ear effusion is effective for the detection of pathogens in patients with OME. While bacteria were thought to be the causative pathogen, this study suggests the etiological role of fungi in the pathogenesis of OME. However, the relationship between fungi and OME requires further studies.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Otitis / Otitis Media / Aspergillus niger / Streptococcus / Bacterias / Basidiomycota / Ventilación / Levaduras / Otitis Media con Derrame / Paecilomyces Tipo de estudio: Estudio diagnóstico / Estudio de incidencia / Estudio pronóstico Límite: Niño / Humanos Idioma: Coreano Revista: Korean Journal of Otolaryngology - Head and Neck Surgery Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Otitis / Otitis Media / Aspergillus niger / Streptococcus / Bacterias / Basidiomycota / Ventilación / Levaduras / Otitis Media con Derrame / Paecilomyces Tipo de estudio: Estudio diagnóstico / Estudio de incidencia / Estudio pronóstico Límite: Niño / Humanos Idioma: Coreano Revista: Korean Journal of Otolaryngology - Head and Neck Surgery Año: 2008 Tipo del documento: Artículo