Establishment of an anxiety-like gastric hypersensitivity rat model of functional dyspepsia induced by a new method of sequential stress / 中国实验动物学报

ABSTRACT

Objective To establish an anxiety-like gastric hypersensitivity ( GHS) rat model of functional dyspep-sia induced by new sequential stress. Methods Twenty-six male 1-day-old Sprague-Dawley rat pups were randomly di-vided into control and model groups ( n=13 in each group) . The model rats received sequential stress from postnatal day 2:neonatal maternal separation (NMS), acute gastric irritation (AGI) and restraint stress (RS). The control rats were reared freely with their mothers without undergoing the sequential stress. From postnatal 8th week all rats started to receive elevated plus maze ( EPM ) , open field test ( OF ) , abdominal withdrawal reflex ( AWR ) and electromyographic test ( EMG) . Results EPM and OF experiments depicted that the model rats showed obvious anxiety-like behavior ( P<0. 05 or P<0. 01). AWR and EMG tests exhibited that the model rats had elevated gastric hypersensitivity to gastric distention (P<0. 05 or P<0. 01). Conclusions An anxiety-like GHS rat model of functional dyspepsia can be successfully estab-lished with our new method of sequential stress.