Study on the effect and mechanism of total flavones of plantago asiatica for rats with calcium oxalate stone in kidney / 中国生化药物杂志

ABSTRACT

Objective To explore the effect and mechanism of total flavones of plantago asiatica for rats with calcium oxalate stone in kidney. Methods 50 rats were divided into control group, model group, total flavones of Plantago asiatica high dose group, total flavones of Plantago asiatica low dose group and positive drug group. Made the model rats with calcium oxalate stone in kidney. Rats in total flavones of plantago asiatica group were intervened by total flavonoids of plantago asiatica, the positive drug group was given puerarin intervention and continuous intervention for 28 days. The pathology of the kidneys, the levels of oxalate and Ca2 in urine, the superoxide dismutase (SOD) and malondialdehyde (MDA) in serum, and osteopontin in the kidneys were examined. Results There were dilated renal tubules and necrosis of renal tubular epithelial cells in total flavones of plantago asiatica group, but the extent of renal tubular cells was significantly improved. The urine oxalate and Ca2 levels, serum MDA levels, and osteopontin levels in the kidney in the total flavonoids of Plantago high dose group, the total flavonoids of Plantago low dose group, positive drug group were significantly lower than that in model group (P<0.05), and serum SOD level was significantly higher than that in model group (P<0.05) . The urine oxalate and Ca2 levels, serum MDA levels, and osteopontin levels in the kidney in the total flavonoids of Plantago high dose group were significantly lower than that in the total flavonoids of Plantago low dose group (P<0.05), and serum SOD level was significantly higher than that in the total flavonoids of Plantago low dose group (P<0.05) . There was no significant difference between the total flavonoids of Plantago high dose group and the positive drug group for above indexes. Conclusions The total flavonoids of pantago asiatica can inhibit the formation of renal calcium oxalate stone, and its mechanism may be the antioxidant stress and the inhibition of osteopontin expression.