Effects of quercetin on proliferation of hepatocarcinoma cell line HepG2 by up-regulating miR-199a expression / 中国生化药物杂志

ABSTRACT

Objective To study the effect of quercetin on proliferation of hepatocarcinoma cells and the expression of miR-199a. Methods Hepatocellular carcinoma cell line HepG2 cells were transfected with 20 μm/L quercetin. MTT proliferation assay were conducted to compare the proliferation, and Annexin V/PI apoptosis assay were adopted to compare the apoptosis of cells in quercetin group and NC group at 12 h, 24 h, 36 h and 48 h. The expression of miR-199a were determined by RT-qPCR and western blotting assays were used to detect the Western blotting was used to detect the expression of VEGFA, Bax and Caspase3. Results MTT showed that quercetin inhibited the proliferation of HepG2 cells (P<0.01). The apoptosis of hepatocellular carcinoma was markedly increased after treated with quercetin (P<0.01). Moreover, quercetin can up-regulate the expression of miR-199a in HepG2 cells. The quercetin decreased the expression of VEGFA, increased the expression of Bax and Caspase 3 on both mRNA and protein level (P<0.01). Conclusion Quercetin can inhibit the proliferation and promote apoptosis of hepatocarcinoma cells by up-regulating the expression of miR-199a.