Molecular mechanism of paeoniflorin regulating transforming growth factor-β in inhibiting the epithelial-mesenchymal transition of glioma U87 cells / 中华行为医学与脑科学杂志

ABSTRACT

Objective To investigate the effect of paeoniflorin on epithelial-mesenchymal transition (EMT),migration and invasion of human glioma U87 cells and its underlying molecular mechanism.Methods Glioma U87 cells were treated with different dose of paeoniflorin.CCK-8 was used to measure the survival rate of glioma cells.Glioma U87 cells were infected with carrying transforming growth factor-β(TGF-β)lentivirus to establish the stable overexpression TGF-β cell line.Wound healing assay and transwell assay were performed to measure cell migration and invasion ability,respectively.Western blot was applied to examined the expression of related proteins.Results Treatment with proliferation of 5 mmol/L and 10 mmol/L had no significant effect on cell survival of U87 cells ((96.00± 3.61) ％,(92.33± 4.04) ％;P5 =0.593,P10 =0.284).But when the dose was up to 15 mmol/L,the survival rate of U87 cells decreased to(75.67±4.58)％,P=0.008.In addition,paeoniflorin inhibited the migration ability of U87 cells and the wound healing rate was(68.20±4.39) ％,(37.70±7.01) ％ when incubated with 5 mmol/L and 10 mmol/L.Similarly,paeoniflorin suppressed invasion ability of U87 cells and the average number of infiltrated cells was (237.67±24.00),(130.67± 32.65),(59.67± 18.15) respectively (P5 =0.002,P10 ＜ 0.01).What's more,paeoniflorin down-regulated the expression of MMP2,MMP9 and epithelial-mesenchymal transition-related proteins (P＜0.05).Overexpression of TGF-β reversed the effect of paeoniflorin on migration,invasion and epithelial-mesenchymal transition of glioma U87 cells.Conclusion Paeoniflorin suppress TGF-β-induced migration,invasion and epithelial-mesenchymal transition in glioma U87 cells.