Autologous periosteum patch enhances tendon-bone healing in rotator cuff repair / 中国组织工程研究

ABSTRACT

BACKGROUND: The latest research shows that the rotator cuff patch can reduce tendon-bone tension, provide the point of attachment for growth factor and cell growth, and promote biological reconstruction of the rotator cuff, but what kind of material as the rotator cuff patch is the most effectively and safety is unclear. OBJECTIVE: To investigate the effect of autologous periosteum patch on the tendon-bone healing in rotator cuff repair.METHODS: Forty-eight New Zealand white rabbits were selected to make acute unilateral rotator cuff injury models and then randomly divided into two groups control group treated with simple suture repair and experimental group treated with autologous periosteum patch on the basis of suture repair. At 4, 8, 12 weeks after repair, proximal humerus specimens were taken, and the histological observation and biomechanical testing were recorded.RESULTS AND CONCLUSION: (1) Histological observation At 4 weeks after repair, the experimental group showed a large amount of interconnected collagen fibers and infiltrated inflammatory cells and a small amount of cartilage cells, and mesenchymal stem cells proliferated remarkably. At 8 weeks after repair, the experimental group showed a large amount of cartilage cells. At 12 weeks, in the experimental group, the tendon bone interface was similar to normal with regular arrangement of cartilage cells. In the control group, loose connective tissues were seen at 4 weeks; a large amount of fibroblasts appeared at the ending point, a small amount of collagen fibers were connected and a small amount of chondrocytes were formed at 8 weeks after repair; collagen fibers were connected and chondrocytes were disordered at 12 weeks after repair. (2) Biomechanical test The maximum load of the two groups increased gradually with time, and the maximum load of the experimental group was higher than that of the control group at different time points (P < 0.05). To conclude, the autologous periosteum patch can effectively promote the tendon-bone healing in rotator cuff repair.