Simultaneous Determination of 16 Kinds of Mycotoxins in Common Dried Fruits by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry / 分析化学

ABSTRACT

A method for simultaneous detection of multi-mycotoxins in ten kinds of common dried fruits was been exploited by using ultra performance liquid chromatography-tandem mass spectrometry. The edible part of dried fruits was ground up with certain quantity of water. The mash was extracted by 10 mmol/L citric acid-acetonitrile, purified by C18 column, and filtered using 0. 22-μm organic filtration. All the sixteen mycotoxins were well separated in 8 min on ACQUITY UPLC BEH C18 column with acetonitrile as phase A and 10 mmol/L ammonium acetate solution containing 0. 1% formic acid as mobile phase B. Result showed that the limits of detection ( LODs) for the sixteen mycotoxins were 0. 01-1. 00 μg/L, with linearity range of 1-200 μg/L and correlation coefficients above 0 . 9981 . The average recoveries of the sixteen mycotoxins in ten matrices were 70. 48% -118. 85%, and the relative standard deviation ( RSD, n=6 ) was 0. 3% -11. 9%. This economical, fast, simple and efficient method could be used for simultaneous detection of multi-mycotoxins in different dried fruits matrixes.