Expression of Toll Like Receptor 2,4 in PBMC Cells of Children with Henoch Schonleinpurpura and Its Relationship with T-cell Subgroups / 现代检验医学杂志

ABSTRACT

Objective To analyze the expression of toll like receptor 2/4(TLR2,TLR4)in peripheral blood mononuclear cells (PBMC)cells of children with henoch schonlein purpura(HSP),and investigate its relationship with T-cell subgroups. Methods A total of 85 HSP children treated in Shanghai Children's Medical Center from March 2015 to February 2017 were selected,including 49 children without renal failure(HSP group)and 16 children with renal failure(HSPN group).30 healthy children were enrolled as control group.The expression of TLR2,TLR4 was examined by real-time PCR.The ratio of Th1,Th2,Th17,Treg,Th1/Th2 and Th17/Treg was detected by flow cytometry.The relationship between TLR2,TLR4 and Th1/Th2,Th17/Treg was analyzed.Results The expression of TLR2 in HSPN,HSP and control group were 2.13± 0.36,1.64±0.27 and 1.05±0.12,the expression of TLR4 were 2.21±0.45,1.82±0.34 and 1.13±0.17 respectively.The differences were statistically significant(F=129.630,81.241,all P<0.01).The percentage of Th1,Treg cells and the ratio of Th1/Th2 in HSPN group were significantly lower than those in HSP group and control group,and the differences were statistically significant(F=34.103,63.828,91.432,all P<0.01).The percentage of Th2,Th17 cells and the ratio of Th17/Treg in HSPN group were significantly lower than those in HSP group and control group,and the differences were statisti-cally significant(F=48.559,48.475,34.909,P<0.01).The expression of TLR2 and TLR4 were negatively correlated with Th1/Th2(r=-0.601,-0.652,P<0.01),and positively correlated with Th17/Treg(r=0.617,0.712,P<0.01).Conclu-sion The expression of TLR2,TLR4 increases in HSP children,and may be related to the imbalance of Th1/Th2 and Th17/Treg immune response.