Therapeutic effect of edaravone combined with rapamycin on neural injury in rats with subarachnoid hem-orrhage / 实用医学杂志

ABSTRACT

Objective To investigate the therapeutic effect of edaravone(Ed)combined with rapamycin (RAP)on neural injury in rats with subarachnoid hemorrhage(SAH),and to provide the evidence for clinical medication. Methods 100 male Sprague-Dawley rats were randomly divided into the following 5 groupssham operated(Sham)group,SAH group,RAP group,Ed group and RAP+Ed group. A rat model of SAH was estab-lished by using the 2 injection of the classic pillow.Learning and memory function assessment was performed by us-ing Morris water maze. The morphological changes of neurons in hippocampus were observed by using optical mi-croscopy. The MDA content was measured by the thiobarbituric acid method. The expressions of autophagy marker proteins(Beclin-1 and LC3-Ⅱ)were detected by immunohistochemistry assay.Results The number of dead neu-rons,MDA content,the expressions of Beclin-1 and LC3-Ⅱwere higher in SAH group than those in Sham group, but learning and memory function index was worse than that in Sham group(P<0.05).The number of dead neu-rons,MDA content in RAP or Ed group were lower than those in SAH group(P < 0.05,respectively),while the expressions of Beclin-1 and LC3-Ⅱwere higher and learning and memory function index was better in RAP or Ed group than those in SAH group(P<0.05,respectively).The number of dead neurons,MDA content in RAP+Ed group were lower than those in RAP or Ed group(P < 0.05,respectively),but the expressions of Beclin-1 and LC3-Ⅱwere higher and learning and memory function index was better in RAP+Ed group than those in RAP or Ed group(P < 0.05,respectively). Conclusions Edaravone combined with RAP had better therapeutic effect on neural injury in rats with SAH,which was associated with enhancing the autophagy of neural cells.