The effect of TXNIP in renal tubular cell injury induced by renal ischemia-reperfusion / 实用医学杂志

ABSTRACT

Objective To investigate the effect of TXNIP in renal ischemia-reperfusion injury induced apoptosis in human kidney proximal tubular cell line. Methods To build an injury model of HK-2 cells in vitro. The level of ROS and rate of apoptosis were analyzed by flow cytometry. The expression levels of proteins were observed by Western Blot. The expression level of TXNIP mRNA was observed by RT-qPCR. Results Compared with the control group,the level of ROS,the rate of cell apoptosis,the expression of cleaved caspase-3 significantly increased(P<0.01),and the expression of GPX4 significantly decreased in HK-2 cells in the OGD/R group(P<0.01).Compared with the OGD/R group,the expression of TXNIP protein and mRNA significantly decreased(P<0.01),the level of ROS,the rate of cell apoptosis significantly decreased(P<0.01),and the expression of GPX4 significantly increased in HK-2 cells in the siRNA TXNIP group(P < 0.01). Conclusion Down regulation of TXNIP can inhibit IRI-induced HK-2 apoptosis through activating GPX4 and decreasing ROS.