Comparison of electrochemiluminescence and ELISA methods in the detection of blood borne pathogens in Gabon
Asian Pacific Journal of Tropical Biomedicine
;
(12): 805-808, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-664241
ABSTRACT
Objective:
To assess the performances of Cobas 6000 e601 and EVOLIS BioRad in the detection of HIV,HBV and HCV in blood donors in Libreville (Gabon).Methods:
A cross-sectional investigation was conducted in July 2017 in a total of 2 000 blood donors recruited at the National Blood transfusion Center,Libreville Gabon.Among them,363 donors were selected to compare the performances of COBAS 6000 e601 (electro-chemiluminescence) and EVOLIS BioRad in detecting HIV,HBV and HCV using Cohen's kappa coefficient.Results:
Both methods yielded similar results for the detection of HIV and HBsAg.A very good agreement of 93.39% and an excellent agreement of 98.90% were obtained for the detection of HIV and HbsAg,with kappa values of 0.80 and 0.98,respectively.The observed agreement of 91.86% was found for the detection of HCV,which gave a fair agreement between the two methods with kappa =0.33.Conclusions:
The two evaluation methods showed a similar performance in the detection of HIV,HBV.However,given the high rate of intra and inter-genotypes recombination known for HIV and HBV,more robust techniques of detection such as polymerase chain reaction should be used to prevent post-transfusion contaminations.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Asian Pacific Journal of Tropical Biomedicine
Año:
2017
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS