Nf1 and BDNF Dysregulation in the Prefrontal Cortex Involved in the Pathogenesis of Attention Deficit Hyperactivity / 医学研究杂志

ABSTRACT

Objective To investigate the interaction between the Nf1 (Neurofibromin 1)and the BDNF (brain derived neurotrophic factor) in the mechanism of attention deficit hyperactivity disorder (ADHD).Methods Spontaneously hypertensive rats were chosen as the experimental group and WKY servesd as control.RT-PCR,Western-blot were used to detect the mRNA and protein expression levels of Nf1 and BDNF.To explore their relationship,BDNF expression levels were detected after Nf1 expression plasmid transferred and transfection in PC12H and CBRH-7919 cells.Results were quantified by Image-pro plus software.Results In the PFC of ADHD model rats SHR,Nf1 expression was abnormally degressive(P =0.000),in common with the expression of BDNF(P =0.000).Ectopic expression of Nf1 further encouraged the expression of BDNF (PC12HP =0.000,7919P ＜0.05).Conclusion Nf1 expression was significantly lower in PFC of SHR than control,and positively correlated with the levels of BDNF.These findings show that in the prefrontal cortex,Nf1-BDNF dysregulation may be involved in the pathomechanism of ADHD.