Salvianolic acid B ameliorates cardiac hypertrophy in T2 DM mice by activating PPARα / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 97-102, 2018.
Article
en Zh
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ABSTRACT
Aim To investigate the protective role of salvianolic acid B ( Sal B ) on cardiac hypertrophy in type 2 diabetes mice , and to explore its influence on peroxisome proliferator activated receptors-α( PPARα) .Methods The type 2 diabetes melitus ( T2DM) mouse model was established by 4 weeks ' high fat diets feeding and one time STZ intraperitoneal injection .The animals were randomly divided into:control, T2DM, T2DM+SalB(100 mg· kg -1 · d-1 ) and Sal B(100 mg· kg -1 · d-1 ) groups.Eight weeks later, heart weight, tibial length, cross section area of cardiomyocytes , protein expression of PPARαin heart tissue were recorded .In vitro, high glucose and high insulin ( HGI ) were used to induce hypertrophic growth in cultured neonatal rat cardiomyocytes ( NRC-Ms) .And cell surface area , 3 H-leucine incorporation , 3 H-D-glucose uptake and PPARαprotein level were measured to observe the effect of Sal B and MK 886, a PPARαinhibitor.Results In T2DM model mice, Sal B could lower heart weight/tibial length and cross sec-tion area of cardiomyocytes , while PPARαprotein level in hearts were improved .In cultured cardiomyocytes , Sal B ( 10 ~100 μmol · L-1 ) ameliorated the in-creased levels of cell surface area ,3 H-leucine incorpo-ration and improved the decreased 3 H-D-glucose up-take and PPARαexpression induced by HGI . But those function could be abolished by MK 886.Conclu-sion Sal B ameliorates cardiac hypertrophy in T 2DM mice, which may be related to its function on PPARαactivation .
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Zh
Revista:
Chinese Pharmacological Bulletin
Año:
2018
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Article