Identification of guanine nucleotide exchange factor ARHGEF 10 and its relationship with tumor malig-nant phenotype / 实用肿瘤学杂志

ABSTRACT

Objective The guanine exchange factors( GEFs) of Dbl family is a major regulatory unit for the malignant transformation of Rho family proteins. It plays a role by converting Rho protein from inactive GDP form to GTP form of Rho protein. In this paper,we discuss the structure and function of a GEF molecule-ARH-GEF 10,and discuss its role in the process of tumor development. Methods The expression of ARHGEF 10 in 42 normal tissues was measured by Real-Time PCR. GST-pulldown technique was used to detect the GEF ac-tivity of ARHGEF 10 in vivo. The transcription factor activity of downstream small molecules was detected by dual-luciferase report gene assay. The high expressive effect of ARHGEF 10 on normal cytoskeleton morphology was performed by dual immunofluorescence staining labeling method. High expressive effects of ARHGEF 10 on cell proliferation,invasion and tumorigenic ability in vitro were examined using CCK8,Transwell and soft agar clony formation assays. Results ARHGEF 10 has a typical GEFs structure,which binds to RhoA in vitro and promotes the proliferation and invasion of NIH3T3 cells,and has significant ability to clone in vitro. Conclusion ARH-GEF 10 is a typical family molecule of guanosine exchange factor that activates RhoA of Rho family,which has obvious oncogene characteristics.