Effect of Taohe Chengqi Decoction on Intestinal Function of Rats with Severe Acute Pancreatitis / 广州中医药大学学报

ABSTRACT

Objective To investigate the effect and mechanism of Taohe Chengqi Decoction(TCD)on intestinal function of severe acute pancreatitis (SAP) rats. Methods Fifty SD rats were randomly divided into blank control group, SAP model group, TCD group (in the dosage of 23.5 mL·kg -1·d -1 by gastric gavage), Octreotide group(in the dosage of 10 μg·kg-1·d-1 by intraperitoneal injection),combined treatment group(TCD in the dosage of 23.5 mL·kg -1·d -1 by gastric gavage, Octreotide in the dosage of 10 μg·kg -1·d -1 by intraperitoneal injection). Except for the blank control group,the rats in the other groups were injected with L-arginine intraperitoneally to establish SAP model. The rats were sacrificed 24 h after the model was successfully established. The serum level of amylase(AMY) in rats was detected by biochemical method. The serum levels of interleukin (IL)-6, IL-10 and tumor necrosis factor (TNF)-α in the fecal flora of rats were measured by enzyme-linked immunosorbent assay (ELISA),and the pathological changes of colorectal tissues and pancreas tissues were observed by hematoxylin-eosin (HE)staining. Results Compared with the blank control group,the levels of serum AMY,TNF-α,IL-6 and IL-10 in SAP model group were significantly increased (P < 0.05), enterococcus and enterobacteria count were increased, while lactobacillus and bifidobacteria count were decreased;the histological structure of pancreas tissues and large intestinal tissues was destroyed, and the proliferation of large amount of inflammatory cells was present. Compared with the SAP model group, serum AMY,TNF-α,and IL-6 levels were decreased and IL-10 level was increased in the three medication groups (P<0.05);enterococcus and enterobacteria count were decreased,while lactobacillus and bifidobacteria count were increased;the pathological features of pancreas tissues and large intestinal tissues were improved;the effect of the combined treatment group was the strongest. Conclusion TCD can improve the intestinal function and intestinal flora of SAP rats,reduce the inflammatory reaction,and prevent SAP from deterioration.