Effects of calcium ionophore A23187 on proliferation ,cycle and expression of caspase-3 in hepatic stellate cells stimulated by transforming growth factor-β1 / 西安交通大学学报(医学版)

ABSTRACT

Objective To study the effect of calcium ionophore A23187 on the proliferation , cycle and expression of caspase-3 in rat hepatic stellate cells by stimulated transforming growth factor Bata 1 (TGF-β1 ) . Methods Hepatic stellate cells were cultured in 37 ℃ and 50 mL/L CO2 incubator .The cells were divided into 5 groupsblank group ,TGF-β1 (5 ng/mL) group ,TGF-β1 +low-,medium-and high-dose calcium ionophore A23187 groups5 ng/mL TGF-β1 stimulation for 24 h ,and then 1μmol/L ,2μmol/L and 4 μmol/L of calcium ion carrier A23187 was added and treated for 24 h .The cells proliferation was detected by MTT .The cell cycle was detected by flow cytometry and the expression of caspase-3 was detected by immunoblotting .Results Different concentrations of calcium ionophore A23187 could significantly inhibit the proliferation of cells ( P< 0 .05 ) . And the dose of calcium ionophore A23187 increased ;RGR in the low-,medium-and high-dose groups was 85 .93% ,61 .71% ,and 48 .43% (P<0 .05) .There was significant difference between the two groups (P<0 .05) .The higher the dose of calcium ionophore A23187 ,the higher the proportion of G1 phase cells ,the lower the ratio of S+ G2 cells ( P<0 .05) ,with significant difference (P<0 .05) .With the increase of calcium ionophore A23187 concentration ,the expression of caspase-3 protein increased significantly ( P< 0 .05 ) . Conclusion Calcium ionophore A23187 prevents hepatic stellate cells from G1 phase to S phase and G2 phase ,inhibits its proliferation and up-regulates the expression of caspase-3 .