Effect of simvastatin preconditioning on acute renal injury in septic rats / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the effect of simvastatin preconditioning on acute renal injury in septic rats.Methods A total of 128 pathogen-free healthy female Wistar rats,aged 49-63 days,weighing 200-250 g,were divided into 4 groups using a random number tablecontrol group (group C,n=8),sham operation group (group Sham,n=40),sepsis group (group Sep,n =40) and simvastatin preconditioning group (group SP,n=40).Sepsis was induced by cecal ligation and puncture (CLP).Simvastatin was injected through a gastric tube into the stomach once a day for 2 consecutive weeks before CLP in group SP,while the equal volume of normal saline was given instead in the other groups.Eight rats were randomly selected from Sham,Sep and SP groups,and the survival rates within 48 h after CLP were recorded.Eight rats were randomly selected at 3,6,24 and 48 h after CLP for blood sampling in Sham,Sep and SP groups,and blood samples were collected after anesthesia in group C.White blood cells were counted,and the serum cystatin C (Cys-C) concentrations were determined by enzyme-linked immunosorbent assay.Kidney specimens were obtained for determination of the percentage of C-reactive protein (CRP) positive cells in renal tissues by immunohistochemistry.The rectal temperature was recorded.Results Compared with group C,the survival rates,white blood cell count and rectal temperature were significantly decreased,and the serum Cys-C concentration and percentage of CRP positive cells in renal tissues were increased in Sep and SP groups (P＜0.05),and no significant change was found in the parameters mentioned above in group Sham (P＞0.05).Compared with group Sep,the survival rates,white blood cell count and rectal temperature were significantly increased,and the serum Cys-C concentration and percentage of CRP positive cells in renal tissues were decreased in group SP (P＜0.05).Conclusion Simvastatin preconditioning can inhibit inflammatory responses of renal tissues and reduce acute renal injury in septic rats.