Effect of High Glucose on MUC5B Expression in Human Airway Epithelial Cells
Clinical and Experimental Otorhinolaryngology
;
: 77-84, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-66658
ABSTRACT
OBJECTIVES:
Excessive production of mucus results in plugging of the airway tract, which can increase morbidity and mortality in affected patients. In patients with diabetes, inflammatory airway disease appears with more frequent relapse and longer duration of symptoms. However, the effects of high glucose (HG) on the secretion of mucin in inflammatory respiratory diseases are not clear. Therefore, this study was conducted in order to investigate the effect and the brief signaling pathway of HG on MUC5B expression in human airway epithelial cells.METHODS:
The effect and signaling pathway of HG on MUC5B expression were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR, enzyme immunoassay, and immunoblot analysis with specific inhibitors and small interfering RNA.RESULTS:
HG increased MUC5B expression and epidermal growth factor receptor (EGFR) expression, and activated the phosphorylation of EGFR and p38 mitogen-activated protein kinase (MAPK). Pretreatment with EGFR inhibitor significantly attenuated the HG-induced phosphorylation of p38 MAPK, and pretreatments with p38 inhibitor or EGFR inhibitor significantly attenuated HG-induced MUC5B expression. In addition, knockdown of p38 MAPK by p38 MAPK siRNA significantly blocked HG-induced MUC5B expression.CONCLUSION:
These findings suggest that HG induces MUC5B expression via the sequential activations of the EGFR/p38 MAPK signaling pathway in human airway epithelial cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
Proteínas Quinasas
/
Recurrencia
/
Mortalidad
/
Técnicas para Inmunoenzimas
/
ARN Interferente Pequeño
/
Proteínas Quinasas p38 Activadas por Mitógenos
/
Células Epiteliales
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Receptores ErbB
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Clinical and Experimental Otorhinolaryngology
Año:
2017
Tipo del documento:
Artículo
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