Serum levels of miR-661 and its clinical value in type 2 diabetes patients with or without microvascular complications / 临床检验杂志

ABSTRACT

Objective To investigate the serum levels of miR-661 in type 2 diabetes(T2DM)patients and type 2 diabetes with microvascular complications(T2DMC),and to further evaluate its clinical auxiliary diagnosis significance.Methods The serum levels of miR-661 were examined in 60 T2DM patients,60 T2DMC patients and 60 healthy controls using quantitative real-time PCR (qRTPCR).The sera levels of biochemical parameters including fasting plasma glucose (FPG),cholesterol (TC),triglycerides (TG),low density lipoprotein (LDL-C),high density lipoprotein (HDL-C) and haemoglobinA1C (HbA1 c) were determined by biochemical analyzer.The ROC curve analysis,correlation analyses and logistic regression analyses were performed to evaluate the clinical auxiliary diagnosis value of serum miR-661,respectively.Results The qRT-PCR results showed that the concentrations of miR-661 was 118 (63.6,192.4) fmol/L in healthy controls,which were significant lower than those of T2DM patients [230.1 (83.6,426.2) fmol/L,(U =1 207,P ＜ 0.01)] and T2DMC patients [441.3 (212.9,1 021) fmol/L,(U =435,P ＜ 0.01)].ROC curve (AUCROc) of miR-661 was 0.665(95％CI0.585 ～0.765,P ＜0.01)for T2DM patients and 0.879(95％CI0.821 ～ 0.938,P＜0.01)for T2DMC patients.The AUCROc between T2DM and T2DMC patients was 0.694 (95 ％ CI0.600 ～ 0.788,P ＜ 0.01).The correlation analyses revealed that the levels of miR-661 was positively correlated with concentrations of FPG(r =0.291,P ＜ 0.05)and LDL-C(r =0.149,P ＜ 0.05),and negatively correlated with concentrations of HDL-C (r =-0.243,P ＜ 0.01).Logistic regression analysis revealed that serum miR-661 was an independent risk factor in T2DM and T2DMC(P ＜ 0.01).Conclusion Serum miR-661 was potential biomarker and risk factor for T2DM and T2DMC.