Progress of liquid chromatography tandem mass spectrometry for the analysis of human steroid hormones / 中华检验医学杂志

ABSTRACT

Steroid hormones are fat soluble molecules produced by cholesterol through a series of enzymatic catalysis.These low-level steroid hormones in body fluids generally play an important role in the regulation of metabolism and sexual function.Therefore,accurate quantification of these hormones is crucial for both diagnosis and treatment of endocrine disorder disease.At present, automated immunoassays are commonly used for the measurement of steroid hormone.However,only one analyte can be detected in one assay.More importantly, a lot of steroid hormones still cannot be detected by immunoassays due to the limitation of specific antibody.Liquid chromatography tandem mass spectrometry(LC-MS/MS)has high sensitivity and high specificity,and can simultaneously detect a variety of steroid hormones in one injection. LC-MS/MS has become a hot research topic in clinical research.