Emodin Inhibits the Angiogenesis of Pancreatic Cancer by Regulating the TGF-β1 and Smad4 / 医学研究杂志

ABSTRACT

Objective To investigate whether emodin suppresses angiogenesis in pancreatic cancer.Methods A nude mouse pancreatic cancer xenograft model was established with SW1990 human pancreatic cancer cells by surgical orthotopic implantation.Different doses of emodin were injected into the abdominal cavities of the tumor-bearing mouse models and controls 3 times weekly for 2 weeks.The expression of CD34 was detected by immunochemistry,and microvessel density was calculated.Quantitative RT-PCR and Western blot were performed to determine the mRNA and protein expressions of TGF-β1 and Smad4.Results A negative dose-dependent association was found among emodin treatments about the weight of tumors.Emodin was associated with lower levels of TGF-β1 mRNA and protein,and higher levels of the mRNAs and proteins Smad4.Conclusion Emodin may repress angiogenesis in pancreatic cancer by altering activities of the TGF-β1 and Smad4.