Effect of DNA Methyltransferase Inhibitor SGI-1027 on Proliferation and Apoptosis of Human Hepatocellular Carcinoma Cells / 中国医科大学学报
Journal of China Medical University
;
(12): 807-811, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-668256
ABSTRACT
Objective To elucidate the inhibitory effect of SGI-1027 on cell proliferation and apoptosis of Huh7 cells.Methods Huh7 cells were treated with different concentrations (0,5,10,15,20,25,30,and 35 μ mol/L) of SGI-1027 for 24 h.MTS assay was performed to detect cell proliferation.Huh7 cells treated with 0.1% DMSO were used as the control group,and those treated with 30 μmol/L SGI-1027 as the experimental group.Flow cytometry was performed to study the cell cycle,and Annexin V-FITC/PI detection for studying cell apoptosis.TUNEL staining was performed to observe changes in cell morphology.Results The results of the MTS assay revealed that SGI-1027 significantly inhibited the proliferation of Huh7 cells in a dose-dependent manner,and the IC50 was 27.3 μmol/L.SGI-1027 did not block the cell cycle of Huh7 cells,but induced cell apoptosis in Huh7 cells.The rates of apoptosis were 3.242% ± 0.204% in the control group and 46.57% ± 2.512% in the experimental group (P < 0.05).In the experimental group,typical apoptotic nucleus alterations were observed by fluorescence microscopy after TUNEL staining.The percentage of apoptotic cells was 1.077% ± 0.407% in the control group and 58.24% ± 8.427% in the experimental group (P < 0.05).Condusion SGI-1027 inhibits Huh7 cell proliferation and induces apoptosis in vitro.
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Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of China Medical University
Año:
2017
Tipo del documento:
Artículo
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