Effect of different harvesting and purification methods on autologous adipocyte viability: theoretical research and application advances / 中国组织工程研究

ABSTRACT

BACKGROUND:How to improve the survival rate after fat transplantation and reduce postoperative complications is always a hotspot.OBJECTIVE:To review the research progress concerning the effect of different harvesting and purification methods on adipocyte viability.METHODS:A computer-based search of CNKI,WanFang,VIP,PubMed,Embase,and Elsevier databases was performed for the literatures addressing the effect of different harvesting and purification methods on adipocyte viability in autologous fat transplantation using the keywords of "autologous fat transplantation,fat grafting,harvesting,aspiration,centrifugation,adipocyte graft survival,water jet-assisted liposuction,Nanofat Grafting" in English and Chinese,respectively.Irrelevant and repetitive papers were excluded,and finally 42 papers were included for further analysis.RESULTS AND CONCLUSION:Autologous fat tissue has become the ideal filling material because of its remarkable advantages.Nonetheless,low adipocyte survival rate and post-transplantation complications,such as high absorption rate,probable cyst,and calcification,limit its clinical application.During autologous fat transplantation,multiple factors can affect the survival rate of fat tissues.It can assist surgeons to choose the optimal method to maximize autologous fat survival rate,reduce postoperative complications such as absorption by understanding the effect of different harvesting and purification methods on adipocyte viability.