Isolation, culture and identification of New Zealand white rabbit talar chondrocytes / 中国组织工程研究

ABSTRACT

BACKGROUND:Based on the previous studies,the New Zealand rabbit talar chondrocyts were isolated,cultured and identified in vitro.OBJECTIVE:To explore the isolation,culture and identification of New Zealand rabbit talar chondrocyts in vitro.METHODS:The chondrocyts were isolated from the talar cartilage of New Zealand white rabbits by type Ⅱ collagen enzyme digestion,and then cultured in vitro.The cells were identified by inverted phase contrast microscope,toluidine blue staining and collagen type Ⅱ immunohistochemical staining.RESULTS AND CONCLUSION:Under the inverted phase contrast microscope,most of passaged chondrocytes presented with polygonal or triangle shape and had round or oval nuclei.Toluidine blue staining showed the hyacinthine chondrocytes and blue cellular matrix.Collagen type Ⅱ immunohistochemical staining showed that the chondrocytes appeared with brown granules in the cytoplasm and membrane.To conclude,a system that can isolate,culture and identify talar chondrocytes from New Zealand rabbits is successfully established.Talar chondrocytes at passages 1-3 grow well and have stable biological properties.