Changes of BDNF expression in hippocampus and spinal cord of rats after compression-induced spinal cord injury / 基础医学与临床

ABSTRACT

Objective To observe changes of BDNF expression in hippocampus and spinal cord of rats after com-pression-induced spinal cord injury .Methods The CSCI model was established with a institution-made device . BDNF in astrocyte, neuron, antegrade and retrograde axons were detected by immunofluorescence .The expression changes of BDNF in hippocampus , lesion centre , the superior segment and the inferior segment which were adjacent to the lesion centre were also determined by Western blot methods .Results The fluorescence intensity of BDNF+-GFAP in lesion centre was increased , while BDNF+-Tuj1 was decreased with time after CSCI .BDNF+-NF200 in superior and inferior segment of lesion was increased .The expression of BDNF in hippocampus , the supe-rior segment and the inferior segment close to the lesion centre reached its peak on the 1st day and then decreased after CSCI(P<0.05).The expression of BDNF in lesion centre decreased gradually (P<0.05).Conclusions De-creasing level of BDNF is regular and may lead to neuronal reduction with compressing time going .