Effect of DATS on experimental corneal neovascularization induced by corneal suture in rats cornea / 国际眼科杂志(Guoji Yanke Zazhi)

ABSTRACT

AIM:To study the effect of diallyl trisulfide (DATS) on experimental corneal neovascularization (CNV) in rats induced by corneal suture and detect the expression of vascular endothelial growth factor (VEGF) and p-AKT in rats cornea.METHODS:The rat model of corneal neovascularization (CNV) was induced by corneal suture.Rats were randomly divided into Group A:physiological saline control group containing DMSO (10 rats);Group B:25μmol/L DATS treatment group (10 rats);Group C:50μmol/L DATS treatment group (10 rats);Group D:100μmol/L DATS treatment group (10 rats);Group E:200μmol/L DATS treatment group (10 rats).The occurrence and development of CNV were observed by slit-lamp microscope at 7d after suture,and the area of CNV were calculated.Two weeks later,HE staining was used to observe the pathological organization form of each cornea,and RT-PCR and Western blot were used to detect the expression of VEGF mRNA and protein expression of VEGF and p-AKT between each groups.RESULTS:The blood vessel area of Group C,D and E was compared with that of Group A,the difference was statistically significant (P＜0.05);HE slice showed corneal edema,angiogenesis and inflammation infiltration situation gradually reduced comparing with the Group A,with the increase of concentration of DATS.RT-PCR showed the expression of VEGF mRNA in Group B,C,D,and E decreased compared with the Group A,and the difference was statistically significant (P＜0.05).Western-blot showed that the expressions of VEGF and p-AKT in Group B,C,D and E decreased gradually compared with those in Group A,and the difference was statistically significant (P＜0.05).CONCLUSION:DATS can inhibit corneal neovascularization of the rats induced by suture.Its mechanism may be associated with suppression of VEGF secretion,down-regulation of VEGF and inactivation of p-AKT.