Determination of Baicalin in Kang 601 Mixture by HPLC / 中国药师
China Pharmacist
;
(12): 2180-2182, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-670135
ABSTRACT
Objective:
To establish a method for the determination of baicalin in Kang 601 mixture.Methods:
An HPLC method was adopted. An Agilent Eclipse Plus-C18 column (250 mm × 4. 6 mm, 5 μm) was used at room temperature (25 ℃) and eluted by methanol-0. 1% phosphoric acid solution(4852) with a flow rate of 0. 8 ml·min-1 . The detection wavelength was 280 nm and the injection volume was 20 μl.Results:
A good linear relationship was obtained between the peak area and the concentration of baicalin within the range of 18. 897-188. 975 μg·ml-1(r=0. 999 9). The average recovery was 99. 86%(RSD=0. 32%, n=9). Conclu-sionThe established method is specific, accurate and reproducible, which can be used for the determination of baicalin in Kang 601 mixture.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacist
Año:
2015
Tipo del documento:
Artículo
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