Preparation of Periostin antibody and the expression of Periostin in mice / 实用口腔医学杂志

ABSTRACT

Objective:To prepare a rabbit anti-mouse Periostin IgG antibody and to study the expression of Periostin in mice.Methods:Complete Freund adjuvant with recombinant Periostin was used to immunize rabbits.Whole blood was collected from the animals after immunization for 5 times. Serum was obtained by centrifugation. The specific IgG antibody was purified from the serum by ammonium sulfate. Total protein isolated from mouse liver, brain, kidney and calvaria and periodontal tissue was used to study the expression of Periostin in mice by Western blot, immunohistochemical staining was applied to examine Periostin expression in jaw bone and dental tissues.Results:Anti-Periostin antibody with the titer of 128 was obtained.Western blot showed that there was a specific band near Mr 90 000 in calvaria and periodontal tissues, but not in liver, brain and kidney.By immunohistochemical examination Periostin was detected in both mandibular periosteum and periodontal ligament,but not in cementum, dentin, alveolar bone and dental pulp.Conclusion:Periostin may play a role in bone and periodontal tissue formation.