Simultaneous Determination of Puerarin and Baicalin in Xiao’ er Shuangjie Zhixie Granules by HPLC / 中国药师
China Pharmacist
;
(12): 863-864,865, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-671117
ABSTRACT
Objective:
To establish a method for the determination of puerarin and balcalin in Xiao’ er Shuangjie Zhixie granules by HPLC.Methods:
An Agilent Zorbax SB-C18 column (250 mm × 4. 6 mm,5 μm) was used. The mobile phase A was 0. 1% phos-phoric acid solution and the mobile phase B was methanol with gradient elution. The detection wavelength was 265 nm,the flow rate was 1. 0 ml·min-1 ,the column temperature was 30℃ and the sample size was 20 μl.Results:
The linear range of pueratin was 0. 121-1. 942 μg (r=0. 999 5)and that of balcalin was 0. 230-3. 686 μg(r=0. 999 5). The average recovery was 98. 10% and 98. 20%with RSD of 1. 34% and 1. 11%(n=6), respectively.Conclusion:
The method is convenient and accurate, which can be used in the de-termination of pueraln and balcalin in Xiao’ er Shuangjie Zhixie granules.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacist
Año:
2015
Tipo del documento:
Artículo
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