PCR-RFLP patterns of four isolates of Trichinella for rDNA ITS1 region
The Korean Journal of Parasitology
;
: 43-48, 2001.
Artículo
en Inglés
| WPRIM
| ID: wpr-67268
ABSTRACT
We have studied the genetic differences among four isolates of Trichinella including a new strain of Trichinella spiralis (ISS 623) recently found from a human case who took a badger in Korea. Because they have a different host origin and came from geographically separated regions, we supposed the genetic pattern of the isolates might be different as had been previously reported. It was analysed by PCR-RFLP analysis of the rDNA repeat that can readily distinguish a species or strain from others. Isolated genomic DNA of each isolate of Trichinella larvae was amplified with ITS1 specific primers and digested with restriction endonucleases. The PCR product of ITS1 was confirmed using Southern blot analysis to be a 910 bp fragment. The restriction fragments of each isolate had variable patterns when it was digested with Rsa 1 only. According to the RFLP patterns, the estimated genetic divergence between each isolate was different. In conclusion, four isolates of Trichinella including a new strain of T. spiralis obtained from a Korean patient may have genetic differences in the ITS1 region and the Shanghai isolate was genetically more similar to the Japanese unknown isolate than others in the ITS1 region.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Polimorfismo de Longitud del Fragmento de Restricción
/
ADN Ribosómico
/
Secuencias Repetitivas de Ácidos Nucleicos
/
Reacción en Cadena de la Polimerasa
/
Trichinella spiralis
/
ADN de Helmintos
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2001
Tipo del documento:
Artículo
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