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PREPARATION AND APPLICATION ON MONOCLONAL ANTI-A ANTI-B ANTIBODIES / 中国法医学杂志
Chinese Journal of Forensic Medicine ; (6)1986.
Artículo en Chino | WPRIM | ID: wpr-673184
ABSTRACT
BALB/C mice were immunized by intraperitoneal injection with A-group erythrocytes, B-group erythrocytes and saliva of A-group,B-group secretor. Immune spleen cells were fused with sp2/0 murine myeloma cells. The supernatants from each well were examined for antibodies by hemagglutination using A-,B-, O-group RBC.3 hybridoma cell lines of secreting anti-A antibodies and 3 hybridoma cell lines of secreting anti-B antibodies were established.These hybridoma cells have maintained and produced antibodies possessing higher specificity and titer.The agglutination titer of supernatants reached 2048-fold. These antibodies were used in the field of forensic science.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Journal of Forensic Medicine Año: 1986 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Journal of Forensic Medicine Año: 1986 Tipo del documento: Artículo