Role of gelatinase and interleukin-8 in the pathogenesis of premature rupture of membrane / 中华围产医学杂志

ABSTRACT

Objective To study the role of Gelatinase and interleukin 8 (IL 8) in premature rupture of membrane (PROM). Methods Sixty pregnant women were divided into two groups. In the observation group, were the pregnant women ( n=30 ) complicated with PROM. The women whose membranes ruptured either normally or manually in cesarean delivery were in the control group. Activities of total gelatinases in amniotic fluid were calculated after the experiment of enzyme linked immunosorbent assay (ELISA) with actin labeled gelatin. The electrophoresis of gelatinase was taken according to reference. IL 8 was determined by sandwich ELISA. Results (1) The activity of total gelatinases of amniotic fluid in observation and control groups during the period before labor, latent stage and active stage were (85?10)?g/L?12 h, (91?7)?g/L?12 h, (104?23)?g/L?12 h, (49?15)?g/L?12 h, (58?15)?g/L?12 h and (102?29)?g/L?12 h respectively,the activity of total gelatinases in active stage was significantly higher than that before labor ( P