Genotyping of K15 gene of human herpesvirus 8 in Kaposi's sarcoma / 中华皮肤科杂志
Chinese Journal of Dermatology
;
(12)2003.
Artículo
en Chino
| WPRIM
| ID: wpr-674256
ABSTRACT
Objective To study the distribution of K15 alleles of human herpesvirus 8 (HHV-8) in Kaposi's sarcoma (KS) in Xinjiang,and to investigate the relationship between clinical profiles of KS and alleles of HHV-8 K15.Methods HHV-8 DNA was extracted with phenol-chloroform-isoamyl alcohol from 27 formalin-fixed,paraffin-embedded tissue specimens of KS.The HHV-8 K15 gene was amplified by nested- PCR,and sequenced for the identification of K15 allele.Results HHV-8 DNA could be detected in 22 (81.48%) out of 27 KS patients in Xinjiang.HHV-8 DNA was detected in all 4 patients with AIDS-related KS.Twenty viral strains were identified as P type,including all 4 from the AIDS-related KS patients;two strains were identified as M type,which were all from the classical KS patients.Conclusions In KS,most of HHV-8 K15 alleles are P type,and some are M type.The 4 patients with AIDS-related KS all carried P type of K15 allele.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Dermatology
Año:
2003
Tipo del documento:
Artículo
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