Studies on Characteristics of Heteromyeloma Cell Line HM—KMC1 / 中国免疫学杂志

ABSTRACT

Establishment of an ideal partner is one of the most important subject of human monoclonalantibody researches.At Present,the human-mouse hybrid myeloma cell line has more advan-tages over plasmacytoma and lymphocyte cell lines.In this subject,heteromyeloma cell line HM—KMC1 was derived from fusion by using murine myeloma SP_(2/0)—AG_(14) with healthy adult pe-ripheral blood lymphocytes that was induced to be a 8—Ag resistant and HAT sensitive cell lineafter a serious cultivation in RPMI1640 complete medium containing 8—Ag,then fused with Blymphocytes from human spleen,tonsil,lymph nodes and peripheral blood,respectively,Six fu-sions have been done successfully and the fusion frequency was 41—129/10~7 cells.