Analysis of chromosomal DNA aberrations in Korean cervical squamous cell carcinoma cell lines by CGH / 대한산부인과학회지

ABSTRACT

OBJECTIVE: Comparative genomic hybridization (CGH) is a new fluorescence in situ hybridization (FISH) technique to identify genomic aberrations in cancers. The purpose of this study was to analyze non-random chromosomal DNA aberrations involved in cervical squamous cell carcinoma cell lines from Korean women. METHODS: We analyzed non-random chromosomal DNA aberrations involved in cervical squamous cell carcinoma cell lines from Korean women, SNU-17, SNU-682, and SNU-902 using CGH. RESULTS: Chromosomal DNA gains of 5p, 5q22-q23, 8q11.2-q12, 14q21-qter, and 20 as well as chromosomal DNA losses of 21 were found frequently. Chromosomal DNA gains on chromosome 3q, 6P, 7p13-pter, 9p22-pter, 9q21-qter, 15q21-q22, 17q22-qter, 18p11.3-pter, 18q11.2-q21, 19p13.3-pter, 19q13.2-q13.3, and 22q12-qter, with losses on 4p14-pter, 10p11.2-p13 and 10q24 were observed in 2 of 3 cell lines. CONCLUSION: Non-random aberrations which were disclosed in this study might be candidate regions for the abnormal genes involved in the tumorigenesis of cervical squamous cell carcinomas. Datas about chromosomal aberrations of Korean squamous cell carcinoma cell lines in this study could afford very useful basic information for the development of diagnostic and therapeutic modalties targeting the abnormal genes associated with uterine cervical cancer in Korea.