Induction of prolongation of allograft survival by immunization with immature DCS / 中国免疫学杂志

ABSTRACT

Objective:To prolong the allograft survival time in immunized mice with immature dendritic cell, and to analyze the mechanism of hypo allo immuno responsiveness induced by immature dendritic cells Methods:Based on mouse cardiac allograft model, the survival of cardiac recipients immunized with mature or immature DC were observed Meanwhile the CTL activity of splenocytes from immature DC immunized recipients was detected Results:The survival time of cardiac allograft was substantially prolonged and the mean survival time extended from 9 1?0 73 days to 25 4?4 27 days It became more effective if those immunized mice were treated in combination with adriamycin application, the mean survival time of allograft was extended to 30 5?3 98 days It was proved that the CTL activity in spleen cells from the mice immunized with immature DCs was much lower (specific release was 16 32%) than that from the immunized mice with mature DCs (specific release was 39 58%) Conclusion:Immature DCs could induce prolongation of allograft survival time It may be possible that low CTL activity in recipients immunized with immature DCs promised this prolongation