Hypoxia-induced apoptosis and anti-apoptosis in ovarian cancer cell line SKOV-3ipl cells and its correlation with cell cycle / 中国癌症杂志
China Oncology
;
(12)1998.
Artículo
en Chino
| WPRIM
| ID: wpr-675075
ABSTRACT
Purpose:
To investigate hypoxia induced apoptosis and anti apoptosis in ovarian cancer cell line SKOV 3ipl cells and its correlation with cell cycle changes.Methods:
The monolayer SKOV 3ipl cells plated in culture bottles were placed in an Anaerobic system insulated with 85% N 2,5% CO 2,10% H 2 and very low O 2 concentration (100 ppm). Apoptosis and cell cycles were measured with FITC Annexin V/PI double stain and DNA prep stain Flow cytometry in cells cultured in anaerobic condition.Results:
Apoptosis in cells cultured in hypoxia condition for 0h,8h,16h,24h. were (0.32?0.01)%,(8.29?0.87)%,(4.23?0.75)%,(0.59?0.08)% respectively. Between 8h or 16h and 0h, P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Oncology
Año:
1998
Tipo del documento:
Artículo
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