Expression of membrane complement regulatory protein on the surface of CD16~+monocyte/macrophage and level of sC5b-9 in the urine of patients with glomerulonephritis / 中国免疫学杂志

ABSTRACT

Objective:To investigate the expression of membrane cofactor protein (MCP)?decay accelerating factor (DAF) and homologous restriction factor 20 (HRF 20) on the surface of CD16 +monocyte/macrophage (Mo/M?) in the urine of patients with glomerulonephritis(GN) Methods:The expression of MCP?DAF and HRF 20 on the surface of urinary CD16 +Mo/M? were tested by Flow Cytometry and the levels of urinary sC5b 9 by ELISA in 134 patients with GN that were divided into mini change (MC)?glomerulosclerosis (GS)?membranous nephropathy (MN) and proliferative glomerulonephritis (PGN) Results:The expressions of MCP?DAF and HRF 20 on the surface of urinary CD16 +Mo/M? and the levels of urinary sC5b 9 in the patients with GS ?MN or PGN were significantly higher than those in controls (P