Preparation and identification of human interleukin-15 monoclonal antibody / 中国免疫学杂志

ABSTRACT

Objective:To produced mouse monoclonal antibodies against IL 15 that should be a valuable reagent for diagnosis and investigation into the pathological significance of IL 15 protein expression Methods:rhIL 15 was conjugated to naked bacteria of salmonella typhimurium as immunogen to immunize BALB/c mice The spleen cells were fused with SP2/0 cells using polyethlene glycol, suspended in selective growth medium (HAT) Clones secreting specific monoclonal antibody were screened by indirect enzyme linked immunosorbent assay(ELISA) After several cloning,several hybridoma cell clones stably producing anti IL 15 monoclonal antibody were obtainied The McAb was identified by Western blot with goat polyclonal antibody raised against mouse Results:Four hybridoma cell strains which could produce monoclonal antibodies to IL 15 were obtained The chromosome number of hybridoma cells was between 90 108, there were metacentric chromosome and submetacentric chromosome in hybridoma cells Monoclonal antibodies were able to inhibit and/or neutralize the effects of IL 15, while naked bacteria could not The sensitivity of Dot blot was 1 5 ng Conclusion:Monoclonal antibodies to rhIL 15 have been prepared and characterized