Acceleration of apoptosis by transfection of bak gene in multi-drug resistant (MDR) bladder cancer cells / 中华泌尿外科杂志
Chinese Journal of Urology
; (12)2000.
Article
en Zh
| WPRIM
| ID: wpr-675181
Biblioteca responsable:
WPRO
ABSTRACT
Objective To observe the effects of bak gene on killing MDR bladder cancer cells and to study its mechanisms. Methods Bak gene was transfected into MDR bladder cancer cells by liposome.The mRNA of bak and bcl 2 were detected by in situ hybridization.The protein of bak and bcl 2 were detected by SABC immunohistochemistry. The growth rate of human bladder cancer cells was studied by constructing the growth curve,cell apoptosis being observed by flow cytometry,and the outline of cells observed by fluorescence stain. Results The expression of bak mRNA was positive in EJ/bak cells (64%, P
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Índice:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Urology
Año:
2000
Tipo del documento:
Article