Detection of growth hormone(GH) in urine with immuno-PCR / 中国免疫学杂志

ABSTRACT

Objective:To establish a highly sensitive method by immuno PCR for measurement of GH in Urine.Methods:Sandwich ELISA was operated in PCR tubes which treated with glutarakdehyde,and PCR was tested in the same PCR tube.The PCR production were electrophoresis in Agarose gel and Scan for gel.Results:The results show that ELISA could be correctly reacted in PCR tubes which treated with glutarakdehyde,more than 0.1 pg/ml GH could be detected.Results of Scan showed that the GH values in urine overnight have significantly deference between 30 normal children and 10 GHD patients which were diagnosed by GH provocative test.Conclusion:The sensitivity of Immuno PCR is 10 3～10 4 times higher than that of ELISA,Immuno PCR could be used in detection of GH in urine of children.