Expression of DNAM-1 on T cells subsets from patients with systemic lupus erythematosus / 中国免疫学杂志

ABSTRACT

Objective:To investigate functional of DNAM 1 antigen in T cell subsets activation process from patients with SLE and determine whether they are correlated with the pathogenesis of SLE.Methods:The peripheral blood monouclear cells from 31 SLE and 30 healthy volunteers were stimulated with PHA.Those cells were quantified using flow cytometric analysis with three colour immunofluorescent staining method.The levels of anti dsDNA,complement C 3 and C 4 were measured as well as.Results:The percentages of DNAM 1 positive from SLE on CD + 4 and CD + 8 T cells were increased,compared with that of the controls (P0.05).The DNAM 1 expression on CD + 8 cells from SLE showed relatively high positive correlation with SLEDAI and anti dsDNA antibody and that on CD + 8 T cells have negative correlation with C 3 and C 4 complement(P0.05).Conclusion:There are some abnormal activated T cell subsets in SLE.The expression of DNAM 1 on CD + 4?CD + 8 T cells with active SLE are increased.That on CD + 8 cells are correlated to SLEDAI?anti dsDNA antibody?C 3 and C 4 complement and there is a clinical correlation with it's levels.So DNAM 1 may play an important roles in the mechanisms of immune activation and immunopathology in SLE.