Resistance to CTL-mediated cytotoxicity of target cells transfected with Wee1 / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2001.
Artículo
en Chino
| WPRIM
| ID: wpr-675458
ABSTRACT
Objective:
To study the effect of resistance to CTL mediated cytotoxity of target cells transfected with Wee1 and treated with anti perforin antibodyMethods:
Cytotoxic T lymphocyte was obtained by mixing lymphocyte NIT culture in vitro,and proliferation index (PI) of lymphocytes was detected(MTT method) The recombinant vector pCMV Wee1Hu/GFP was transfected into mammalian cells NIT With the function of anti perforin antibody, NIT and NIT transfected with Wee1 used as target cells, CTL mediated cytotoxity was detected by LDH methodResults:
CTL proliferation was induced by mixing cell culture With anti perforin antibody, the cytolysis quantity of NIT transfected with Wee1 was leastConclusion:
Target cells transfected with Wee1 could resist CTL mediated cytotoxity, and anti perforin antibody obviously increased the effect
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Immunology
Año:
2001
Tipo del documento:
Artículo
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